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1Institute of Immunology,Third Military Medical University, 2Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital,Sun Yat-Sen University, 3Shigatse Branch, Xinqiao Hospital,Third Military Medical University, 4Department of Emergency Medicine, Southwest Hospital,Third Military Medical University, 5Cancer Center,The General Hospital of Western Theater Command
Chapters
- 00:04Introduction
- 00:56Ectopic Tumor Implantation of B16F10-OVA Cells in the Inguinal Region of Mice
- 02:23Adoptive Transfer of Congenically Marked OT-I T Cells into Tumor-Bearing Mice
- 03:26Dissect Tumor Mass from Tumor-Bearing Donor Mice
- 04:31Subcutaneous Transplantation of Donor-Derived Tumor onto the Tumor-Matched Recipient Mice
- 06:14Results: For Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
- 07:04Conclusion
Ici, nous présentons un protocole de transplantation tumorale pour la caractérisation des lymphocytes infiltrés tumoraux inhérents à la tumeur et dérivés de la périphérie dans un modèle tumoral murin. Le traçage spécifique de l’afflux de cellules immunitaires dérivées du receveur avec cytométrie en flux révèle la dynamique des changements phénotypiques et fonctionnels de ces cellules au cours des réponses immunitaires antitumorales.
