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肿瘤移植用于评估小鼠肿瘤浸润CD8 + T细胞的动力学
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JoVE 신문 암 연구학
Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
DOI:

07:36 min

June 12, 2021

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Chapters

  • 00:04Introduction
  • 00:56Ectopic Tumor Implantation of B16F10-OVA Cells in the Inguinal Region of Mice
  • 02:23Adoptive Transfer of Congenically Marked OT-I T Cells into Tumor-Bearing Mice
  • 03:26Dissect Tumor Mass from Tumor-Bearing Donor Mice
  • 04:31Subcutaneous Transplantation of Donor-Derived Tumor onto the Tumor-Matched Recipient Mice
  • 06:14Results: For Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
  • 07:04Conclusion

Summary

자동 번역

在这里,我们提出了一种肿瘤移植方案,用于表征小鼠肿瘤模型中肿瘤固有和外周来源的肿瘤浸润淋巴细胞。通过流式细胞术特异性追踪受体来源的免疫细胞的流入,揭示了抗肿瘤免疫反应期间这些细胞表型和功能变化的动力学。

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