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Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
 

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Article DOI: 10.3791/62569-v 14:30 min August 7th, 2021
August 7th, 2021

챕터

요약

Here, we present an immunophenotyping strategy for the characterization of megakaryocyte differentiation, and show how that strategy allows the sorting of megakaryocytes at different stages with a fluorescence-activated cell sorter. The methodology can be applied to human primary tissues, but also to megakaryocytes generated in culture in vitro.

Tags

Keywords: Immunophenotyping Cell Sorting Human Megakaryocytes Hematopoietic Progenitors Flow Cytometry Megakaryopoiesis Polyploidy Surface Markers Multi-omics Studies Platelet Production
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