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JoVE Journal
Biochemistry

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Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
 

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Article DOI: 10.3791/63118-v 11:44 min January 19th, 2022
January 19th, 2022

챕터

요약

The protocol describes the preparation of cell membrane affinity chromatography (CMAC) columns with immobilized cell membrane fragments containing functional transmembrane tropomyosin kinase receptor B proteins. The use of CMAC columns in the identification of specialized plant metabolites interacting with these receptors and present in complex natural mixtures is also explained.

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Keywords: Cellular Membrane Affinity Chromatography Transmembrane Receptors Plant Metabolites Tropomyosin Kinase Receptor Benzamidine PMSF Protease Inhibitor ATP Tris-HCl Buffer Homogenization Buffer Cell Harvesting Cell Lysis Column Preparation
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