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1Department of Electronic and Computer Engineering,The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience,The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health,The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center,The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences,Southern University of Science and Technology
Chapters
- 00:04Introduction
- 00:59Starting-Up the Lasers
- 02:02Optical Alignment of the Combined TPEF-Stimulated Raman Scattering Microscope
- 04:39Optimizing Imaging Conditions
- 06:36In Vivo TPEF-SRS Imaging of Mouse Spinal Cord
- 08:03Results: In Vivo Dual-Modal Imaging of Spinal Axons and Myelin Sheaths
- 08:31Conclusion
Die stimulierte Raman-Streuung (SRS) -Mikroskopie ermöglicht eine markierungsfreie Bildgebung von Biomolekülen basierend auf ihrer intrinsischen Schwingung spezifischer chemischer Bindungen. In diesem Protokoll wird der instrumentelle Aufbau eines integrierten SRS- und Zwei-Photonen-Fluoreszenzmikroskops beschrieben, um zelluläre Strukturen im Rückenmark lebender Mäuse sichtbar zu machen.
