JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Electronic and Computer Engineering,The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience,The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health,The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center,The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences,Southern University of Science and Technology
Chapters
- 00:04Introduction
- 00:59Starting-Up the Lasers
- 02:02Optical Alignment of the Combined TPEF-Stimulated Raman Scattering Microscope
- 04:39Optimizing Imaging Conditions
- 06:36In Vivo TPEF-SRS Imaging of Mouse Spinal Cord
- 08:03Results: In Vivo Dual-Modal Imaging of Spinal Axons and Myelin Sheaths
- 08:31Conclusion
La microscopía de dispersión Raman estimulada (SRS) permite obtener imágenes sin etiquetas de biomoléculas en función de su vibración intrínseca de enlaces químicos específicos. En este protocolo, se describe la configuración instrumental de un SRS integrado y un microscopio de fluorescencia de dos fotones para visualizar las estructuras celulares en la médula espinal de ratones vivos.
