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Bioengineering

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Pattern Generation for Micropattern Traction Microscopy
 

Pattern Generation for Micropattern Traction Microscopy

Article DOI: 10.3791/63628-v 09:26 min February 17th, 2022
February 17th, 2022

챕터

요약

We describe improvements to a standard method for measuring cellular traction forces, based on microcontact printing with a single subtractive patterning step of dot arrays of extracellular matrix proteins on soft hydrogels. This method allows for simpler and more consistent fabrication of island patterns, essential for controlling cell cluster shape.

Tags

Keywords: Pattern Generation Micropattern Traction Microscopy PDMS Master Mold Coverslips Plasma Treatment Fluorescent Labeled Protein 3-APTMS Glutaraldehyde
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