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Análise da Morfologia Organoide Retal (ADM)
 
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Análise da Morfologia Organoide Retal (ADM): Um Ensaio Diagnóstico na Fibrose Cística

Article DOI: 10.3791/63818-v • 07:56 min • June 10th, 2022
June 10th, 2022 •
1,2, 1, 3,4, 5, 6, 7,8, 7,9, 8, 3,4, 1,2, 1,2, 10,11, 1,2, 1,2
1Department of Development and Regeneration, Woman and Child Unit, CF research lab, KU Leuven, 2Department of Pediatrics, Pediatric Pulmonology, University Hospitals Leuven, 3VIB Bio Imaging Core, VIB-KU Leuven Center for Brain & Disease Research, 4Department for Neuroscience, KU Leuven, 5Interuniversity Center for Biostatistics and Statistical Bioinformatics, University of Leuven and University of Hasselt, 6Department of Pediatrics, 2nd Faculty of Medicine, Charles University and Motol University Hospital, 7Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, 8Department of Development and Regeneration, Stem Cell Institute Leuven (SCIL), KU Leuven, 9Department of Gastroenterology and Hepatology, University Hospitals Leuven, KU Leuven, 10Department of Chronic Diseases, Metabolism and Ageing; Pneumology, KU Leuven, 11Department of Respiratory Diseases, University Hospitals Leuven

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Este protocolo descreve a análise da morfologia organoide retal (ADM), um novo ensaio diagnóstico para fibrose cística (FC). As características morfológicas, nomeadamente a arredondamento (índice de circularidade, IC) e a presença de um lúmen (razão de intensidade, RI), são uma medida da função CFTR. A análise de 189 sujeitos mostrou perfeita discriminação entre FC e não FC.

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Medicina Edição 184
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