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Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria
 

Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria

Article DOI: 10.3791/64147-v • 11:49 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1
1Department of Medicine, Division of Nephrology, The Icahn School of Medicine at Mount Sinai

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요약
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The article describes the method for isolating conditionally immortalized glomerular endothelial cells from the kidneys of transgenic mice expressing the thermolabile simian virus 40 and photo-activatable mitochondria, PhAMexcised. We describe the procedure for glomeruli isolation from whole kidneys using beads, digestion steps, seeding, and culturing of GECs-CD31 positive.

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Keywords: Immortalized Glomerular Endothelial Cells Fluorescent Mitochondria In Vitro Diabetic Kidney Disease Cell Isolation Kidney Glomerular Cells Magnetic Beads Collagen IV Collagenase Type II Cell Culture
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