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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolement et quantification du virus d’Epstein-Barr à partir de la lignée cellulaire P3HR1
 
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Isolement et quantification du virus d’Epstein-Barr à partir de la lignée cellulaire P3HR1

Article DOI: 10.3791/64279-v • 09:14 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1,2
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut

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Ce protocole permet d’isoler les particules du virus d’Epstein-Barr de la lignée cellulaire humaine P3HR1 lors de l’induction du cycle lytique viral avec le phorbol 12-myristate 13-acétate. L’ADN est ensuite extrait de la préparation virale et soumis à une PCR en temps réel pour quantifier la concentration de particules virales.

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Immunologie et infection numéro 187
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