Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Determination of In Vitro and Cellular Turn-on…
JoVE Journal
Biochemistry

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Biochemistry
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
 

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Article DOI: 10.3791/64367-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1
1Department of Chemistry and Henry Eyring Center for Cell & Genome Science, University of Utah
* These authors contributed equally

챕터

요약
Automatic Translation

The protocol presents two methods to determine the kinetics of the fluorogenic RNA aptamers Spinach2 and Broccoli. The first method describes how to measure fluorogenic aptamer kinetics in vitro with a plate reader, while the second method details the measurement of fluorogenic aptamer kinetics in cells by flow cytometry.

Tags

Keywords: Fluorogenic RNA Aptamers In Vitro Kinetics Cellular Kinetics Real-time Studies RNA Renaturation Thermocycler Plate Reader Injector Fluorescence Measurements Binding Buffer D-FHBI
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter