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Following the Dynamics of Structural Variants in Experimentally Evolved Populations
 

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Article DOI: 10.3791/64709-v 04:52 min February 3rd, 2023
February 3rd, 2023

챕터

요약

We developed a cost-effective method to follow non-single nucleotide polymorphism allele dynamics that can easily be adapted to experimental evolution frozen archives. A triplet PCR technique was coupled with automated parallel capillary electrophoresis to quantify the relative frequency of an insertion allele over the course of experimental evolution.

Tags

Keywords: Structural Variants Experimental Evolution Capillary Electrophoresis IS10 Insertion MutS Gene PCR DNA Quantification Calibration Curve Parallel Capillary Electrophoresis
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