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JoVE Journal Neuroscience
In vivo Calciumbilleddannelse af neuronale ensembler i netværk af primære sensoriske neuroner i intakte dorsale rodganglier
 
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In vivo Calciumbilleddannelse af neuronale ensembler i netværk af primære sensoriske neuroner i intakte dorsale rodganglier

Article DOI: 10.3791/64826-v • 09:07 min • February 10th, 2023
February 10th, 2023 •
1, 1, 1, 1, 1,2
1Department of Oral & Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, 2Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences, University of Texas Health Science Center at San Antonio

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Denne protokol beskriver den kirurgiske eksponering af dorsalrodsganglion (DRG) efterfulgt af GCaMP3 (genetisk kodet Ca2+ indikator; Grønt fluorescerende protein-calmodulin-M13-protein 3) Ca2+ billeddannelse af neuronale ensembler ved hjælp af Pirt-GCaMP3-mus, mens der påføres en række stimuli på den ipsilaterale bagpote.

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Denne måned i JoVE nummer 192
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