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JoVE Journal Neuroscience
In Vivo Calciumbeeldvorming van neuronale ensembles in netwerken van primaire sensorische neuronen in intacte dorsale wortelganglia
 
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In Vivo Calciumbeeldvorming van neuronale ensembles in netwerken van primaire sensorische neuronen in intacte dorsale wortelganglia

Article DOI: 10.3791/64826-v • 09:07 min • February 10th, 2023
February 10th, 2023 •
1, 1, 1, 1, 1,2
1Department of Oral & Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, 2Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences, University of Texas Health Science Center at San Antonio

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Dit protocol beschrijft de chirurgische blootstelling van het dorsale wortelganglion (DRG) gevolgd door GCaMP3 (genetisch gecodeerde Ca2 + indicator; Green Fluorescent Protein-Calmodulin-M13 Protein 3) Ca2+ beeldvorming van de neuronale ensembles met behulp van Pirt-GCaMP3-muizen tijdens het toepassen van een verscheidenheid aan stimuli op de ipsilaterale achterpoot.

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Deze maand in JoVE nummer 192
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