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Une méthode de culture 3D de sphéroïdes de lignées cellulaires de poissons-zèbres embryonnaires et hépatiques
JoVE 신문
환경과학
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JoVE 신문 환경과학
A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
DOI:

08:00 min

January 20, 2023

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Chapters

  • 00:05Introduction
  • 00:55Cell Culture Medium and Monolayer Cultures
  • 02:20Cell Counting with Trypan Blue Dye Exclusion Test
  • 03:07Cell Plating in ULA-Plates
  • 04:48Results: Characterization of Spheroids Developed with 3D Spheroid Method
  • 07:12Conclusion

Summary

자동 번역

Ici, nous présentons un protocole de culture 3D efficace, facile et rapide pour la formation de sphéroïdes de deux lignées cellulaires de poisson zèbre (Danio rerio): ZEM2S (embryon) et ZFL (hépatocytes normaux).

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