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Biochemistry

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JoVE Journal Biochemistry
功能性 3D 球状体细胞培养物的半自动表型分析
 
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功能性 3D 球状体细胞培养物的半自动表型分析

Article DOI: 10.3791/65086-v • 06:48 min • August 18th, 2023
August 18th, 2023 •
1, 1, 2,3, 2, 3, 1
1Department of Biochemistry, Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3CelVivo ApS

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我们提出了一种使用图像捕获和蛋白质组学培养高可重复球体及其表型表征的方案。

Tags

生物化学，第 198 期，Clinostat 培养箱，3D 细胞培养，癌肝细胞，非癌性肝细胞，肝细胞，3D 细胞培养箱，细胞生长监测，细胞生长快照，球状体计数，球状体测量，THLE-3 细胞系，HepG2/C3A 细胞系，蛋白质组学实验，细胞信号传导扰动，呼吸链代谢，金属解毒，表型表征，图像捕获，蛋白质组学管道
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