Alan A. Doucette Department of Chemistry Dalhousie University Biography Publications Institution JoVE Articles Alan A. Doucette has not added a biography. If you are Alan A. Doucette and would like to personalize this page please email our Author Liaison for assistance. Publications Maximizing Cumulative Trypsin Activity with Calcium at Elevated Temperature for Enhanced Bottom-Up Proteome Analysis Biology. Oct, 2022 | Pubmed ID: 36290348 Automated Electrokinetic Platform for High-Throughput Sodium Dodecyl Sulfate Depletion Ahead of Proteome Analysis by Mass Spectrometry Analytical Chemistry. 10, 2021 | Pubmed ID: 34644045 Recent Advances in Top-down Proteome Sample Processing Ahead of MS Analysis Mass Spectrometry Reviews. May, 2021 | Pubmed ID: 34047392 Correction To: A Robust Strategy for Proteomic Identification of Biomarkers of Invasive Phenotype Complexed with Extracellular Heat Shock Proteins Cell Stress & Chaperones. Mar, 2021 | Pubmed ID: 33355877 Mass Spectrometry Profiling of Low Molecular Weight Proteins and Peptides Isolated by Acetone Precipitation Analytica Chimica Acta. Nov, 2020 | Pubmed ID: 33161983 Rapid and Quantitative Protein Precipitation for Proteome Analysis by Mass Spectrometry Journal of Proteome Research. May, 2020 | Pubmed ID: 32195589 Developing Front-end Devices for Improved Sample Preparation in MS-based Proteome Analysis Journal of Mass Spectrometry : JMS. Mar, 2020 | Pubmed ID: 31957906 A Robust Strategy for Proteomic Identification of Biomarkers of Invasive Phenotype Complexed with Extracellular Heat Shock Proteins Cell Stress & Chaperones. 11, 2019 | Pubmed ID: 31650515 Membrane-Based SDS Depletion Ahead of Peptide and Protein Analysis by Mass Spectrometry Proteomics. 05, 2018 | Pubmed ID: 29575800 Accelerated SDS Depletion from Proteins by Transmembrane Electrophoresis: Impacts of Joule Heating Electrophoresis. Jun, 2018 | Pubmed ID: 29417596 The Benefits (and Misfortunes) of SDS in Top-down Proteomics Journal of Proteomics. 03, 2018 | Pubmed ID: 28286130 Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by Chaperone Affinity Enrichment Proteomes. Oct, 2017 | Pubmed ID: 28991197 Mass Spectrometry of Intact Proteins Reveals +98 U Chemical Artifacts Following Precipitation in Acetone Journal of Proteome Research. 02, 2017 | Pubmed ID: 28088865 Automated SDS Depletion for Mass Spectrometry of Intact Membrane Proteins Though Transmembrane Electrophoresis Journal of Proteome Research. 08, 2016 | Pubmed ID: 27376408 Preventing N- and O-formylation of Proteins when Incubated in Concentrated Formic Acid Proteomics. Apr, 2016 | Pubmed ID: 26840995 Comparison of Sodium Dodecyl Sulfate Depletion Techniques for Proteome Analysis by Mass Spectrometry Journal of Chromatography. A. Oct, 2015 | Pubmed ID: 26422304 A Two-stage Spin Cartridge for Integrated Protein Precipitation, Digestion and SDS Removal in a Comparative Bottom-up Proteomics Workflow Journal of Proteomics. Apr, 2015 | Pubmed ID: 25316050 Resolubilization of Precipitated Intact Membrane Proteins with Cold Formic Acid for Analysis by Mass Spectrometry Journal of Proteome Research. Dec, 2014 | Pubmed ID: 25384094 Reprint of "GELFrEE Fractionation Combined with Mass Spectrometry for Proteome Analysis of Secreted Toxins from Enteropathogenic Escherichia Coli (EPEC)" Molecular and Cellular Probes. Apr-Jun, 2014 | Pubmed ID: 24486296 Proteomic Analysis of Rat Proximal Tubule Cells Following Stretch-induced Apoptosis in an in Vitro Model of Kidney Obstruction Journal of Proteomics. Apr, 2014 | Pubmed ID: 24316357 Proteomic Workflows for Biomarker Identification Using Mass Spectrometry - Technical and Statistical Considerations During Initial Discovery Proteomes. Aug, 2013 | Pubmed ID: 28250400 Dual LC-MS Platform for High-throughput Proteome Analysis Journal of Proteome Research. Dec, 2013 | Pubmed ID: 24090060 Maximizing Recovery of Water-soluble Proteins Through Acetone Precipitation Analytica Chimica Acta. Sep, 2013 | Pubmed ID: 24016582 GELFrEE Fractionation Combined with Mass Spectrometry for Proteome Analysis of Secreted Toxins from Enteropathogenic Escherichia Coli (EPEC) Molecular and Cellular Probes. Oct-Dec, 2013 | Pubmed ID: 23831145 A Universal, High Recovery Assay for Protein Quantitation Through Temperature Programmed Liquid Chromatography (TPLC) Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Mar, 2013 | Pubmed ID: 23435344 Critical Assessment of the Spectroscopic Activity Assay for Monitoring Trypsin Activity in Organic-aqueous Solvent Analytical Biochemistry. Apr, 2013 | Pubmed ID: 23333224 Chromatographic Behaviour of Peptides Following Dimethylation with H2/D2-formaldehyde: Implications for Comparative Proteomics Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Nov, 2012 | Pubmed ID: 23122402 Perfluorooctanoic Acid and Ammonium Perfluorooctanoate: Volatile Surfactants for Proteome Analysis? Rapid Communications in Mass Spectrometry : RCM. Mar, 2012 | Pubmed ID: 22302492 Intact Proteome Fractionation Strategies Compatible with Mass Spectrometry Expert Review of Proteomics. Dec, 2011 | Pubmed ID: 22087661 Implications of Partial Tryptic Digestion in Organic-aqueous Solvent Systems for Bottom-up Proteome Analysis Analytica Chimica Acta. Oct, 2011 | Pubmed ID: 21889634 A Qualitative Proteome Investigation of the Sediment Portion of Human Urine: Implications in the Biomarker Discovery Process Proteomics. Clinical Applications. Jan, 2009 | Pubmed ID: 21136939 Multiplexed Size Separation of Intact Proteins in Solution Phase for Mass Spectrometry Analytical Chemistry. Aug, 2009 | Pubmed ID: 19572727 Evaluation of a Solution Isoelectric Focusing Protocol As an Alternative to Ion Exchange Chromatography for Charge-based Proteome Prefractionation Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Mar, 2009 | Pubmed ID: 19246255 Rapid and Effective Focusing in a Carrier Ampholyte Solution Isoelectric Focusing System: a Proteome Prefractionation Tool Journal of Proteome Research. Apr, 2008 | Pubmed ID: 18284188 Gel-eluted Liquid Fraction Entrapment Electrophoresis: an Electrophoretic Method for Broad Molecular Weight Range Proteome Separation Analytical Chemistry. Mar, 2008 | Pubmed ID: 18229945 Cyclic Polyamide Oligomers Extracted from Nylon 66 Membrane Filter Disks As a Source of Contamination in Liquid Chromatography/mass Spectrometry Journal of the American Society for Mass Spectrometry. May, 2006 | Pubmed ID: 16517177 Tips And Tricks for Proteome Sample Preparation Alan A. Doucette1 1Department of Chemistry, Dalhousie University JoVE 64830 Editorial Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry Jessica L. Nickerson1, Venus Baghalabadi1, Ziheng Dang1, Victoria A. Miller2, Sara L. Little2, Alan A. Doucette1 1Department of Chemistry, Dalhousie University, 2Proteoform Scientific Inc. JoVE 63503 Química
Tips And Tricks for Proteome Sample Preparation Alan A. Doucette1 1Department of Chemistry, Dalhousie University JoVE 64830 Editorial
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry Jessica L. Nickerson1, Venus Baghalabadi1, Ziheng Dang1, Victoria A. Miller2, Sara L. Little2, Alan A. Doucette1 1Department of Chemistry, Dalhousie University, 2Proteoform Scientific Inc. JoVE 63503 Química