Chad R. Borges School of Molecular Sciences Arizona State University Biography Publications Institution JoVE Articles Chad R. Borges Dr. Borges received a B.S. in Chemistry from Walla Walla College (WA) in 1997. He went on to complete a Ph.D. in Analytical Toxicology (2001) at the University of Utah under Prof. Douglas Rollins, the medical director in charge of doping control for the 2002 Olympic Games. Dr. Borges’ dissertation research focused on the role of hair pigments and cellular transport in drug incorporation into hair. He continued his education as a postdoctoral fellow at Michigan State University under pioneering mass spectrometrist J. Throck Watson, where he studied protein mass spectrometry with an emphasis on characterizing protein posttranslational modifications. In 2003, he returned to Utah where he helped establish the Olympic-level certified Sports Medicine Research and Testing Laboratory. Following successful laboratory accreditation he transitioned to The Biodesign Institute at Arizona State University in 2007 where he worked for several years as a research faculty member at The Biodesign Institute with Dr. Randall Nelson before joining the Department of Chemistry & Biochemistry (now School of Molecular Sciences) in 2013. His current research focuses on the roles of protein post-translational modifications in human health and disease as well as the development of markers and approaches for ensuring biospecimen integrity. Publications Diagnostic and Prognostic Performance of Blood Plasma Glycan Features in the Women Epidemiology Lung Cancer (WELCA) Study Journal of Proteome Research. 11, 2019 | Pubmed ID: 31566983 Delta-S-Cys-Albumin: A Lab Test That Quantifies Cumulative Exposure of Archived Human Blood Plasma and Serum Samples to Thawed Conditions Molecular & Cellular Proteomics : MCP. 10, 2019 | Pubmed ID: 31324658 Behavior of Blood Plasma Glycan Features in Bladder Cancer PloS One. Month, 2018 | Pubmed ID: 30040854 Stage Dependence, Cell-Origin Independence, and Prognostic Capacity of Serum Glycan Fucosylation, β1-4 Branching, β1-6 Branching, and α2-6 Sialylation in Cancer Journal of Proteome Research. 01, 2018 | Pubmed ID: 29129073 Ex vivo Instability of Glycated Albumin: A Role for Autoxidative Glycation Archives of Biochemistry and Biophysics. 09, 2017 | Pubmed ID: 28712850 A Spin Column-free Approach to Sodium Hydroxide-based Glycan Permethylation The Analyst. Jul, 2017 | Pubmed ID: 28635997 Variations in Native Protein Glycation and Plasma Antioxidants in Several Birds of Prey Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology. Aug, 2017 | Pubmed ID: 28529085 Bacterial Expression, Correct Membrane Targeting and Functional Folding of the HIV-1 Membrane Protein Vpu Using a Periplasmic Signal Peptide PloS One. Month, 2017 | Pubmed ID: 28225803 Chicken Albumin Exhibits Natural Resistance to Glycation Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology. Jan, 2017 | Pubmed ID: 27773732 Glycan Structure of Gc Protein-derived Macrophage Activating Factor As Revealed by Mass Spectrometry Archives of Biochemistry and Biophysics. 09, 2016 | Pubmed ID: 27503803 Development of Quantitative Mass Spectrometric Immunoassay for Serum Amyloid A Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals. Dec, 2016 | Pubmed ID: 27308834 Characterization of Additional Vitamin D Binding Protein Variants The Journal of Steroid Biochemistry and Molecular Biology. May, 2016 | Pubmed ID: 26924582 Ebselen Inhibits QSOX1 Enzymatic Activity and Suppresses Invasion of Pancreatic and Renal Cancer Cell Lines Oncotarget. Jul, 2015 | Pubmed ID: 26158899 Gamma-carboxylation and Fragmentation of Osteocalcin in Human Serum Defined by Mass Spectrometry Molecular & Cellular Proteomics : MCP. Jun, 2015 | Pubmed ID: 25855755 Serum Amyloid a Truncations in Type 2 Diabetes Mellitus PloS One. Month, 2015 | Pubmed ID: 25607823 Mass Spectrometric Immunoassay for the Qualitative and Quantitative Analysis of the Cytokine Macrophage Migration Inhibitory Factor (MIF) Proteome Science. Month, 2014 | Pubmed ID: 25328446 Oxidative Protein Folding: Nature's Knotty Challenge Antioxidants & Redox Signaling. Jul, 2014 | Pubmed ID: 24766375 Elevated Plasma Albumin and Apolipoprotein A-I Oxidation Under Suboptimal Specimen Storage Conditions Molecular & Cellular Proteomics : MCP. Jul, 2014 | Pubmed ID: 24736286 Parallel Workflow for High-throughput (>1,000 Samples/day) Quantitative Analysis of Human Insulin-like Growth Factor 1 Using Mass Spectrometric Immunoassay PloS One. Month, 2014 | Pubmed ID: 24664114 The Application of Multiple Reaction Monitoring and Multi-analyte Profiling to HDL Proteins Lipids in Health and Disease. Month, 2014 | Pubmed ID: 24397693 Techniques for the Analysis of Cysteine Sulfhydryls and Oxidative Protein Folding Antioxidants & Redox Signaling. Jul, 2014 | Pubmed ID: 24383618 Mass Spectrometric Immunoassay and MRM As Targeted MS-based Quantitative Approaches in Biomarker Development: Potential Applications to Cardiovascular Disease and Diabetes Proteomics. Clinical Applications. Aug, 2013 | Pubmed ID: 23696124 Multiplexed Surrogate Analysis of Glycotransferase Activity in Whole Biospecimens Analytical Chemistry. Mar, 2013 | Pubmed ID: 23368525 Revealing the Allosterome: Systematic Identification of Metabolite-protein Interactions Biochemistry. Jan, 2012 | Pubmed ID: 22122470 RNA-protein Binding Interface in the Telomerase Ribonucleoprotein Proceedings of the National Academy of Sciences of the United States of America. Dec, 2011 | Pubmed ID: 22123986 Mass Spectrometric Immunoassay Revisited Journal of the American Society for Mass Spectrometry. Jun, 2011 | Pubmed ID: 21953037 Mass Spectrometric Immunoassay of Intact Insulin and Related Variants for Population Proteomics Studies Proteomics. Clinical Applications. Aug, 2011 | Pubmed ID: 21656909 Building Multidimensional Biomarker Views of Type 2 Diabetes on the Basis of Protein Microheterogeneity Clinical Chemistry. May, 2011 | Pubmed ID: 21402800 C-peptide Microheterogeneity in Type 2 Diabetes Populations Proteomics. Clinical Applications. Jan, 2010 | Pubmed ID: 21137020 Intrapersonal and Populational Heterogeneity of the Chemokine RANTES Clinical Chemistry. Sep, 2010 | Pubmed ID: 20802101 Cysteine Sulfenic Acid As an Intermediate in Disulfide Bond Formation and Nonenzymatic Protein Folding Biochemistry. Sep, 2010 | Pubmed ID: 20712299 Possibilities and Pitfalls in Quantifying the Extent of Cysteine Sulfenic Acid Modification of Specific Proteins Within Complex Biofluids BMC Biochemistry. Month, 2010 | Pubmed ID: 20594348 Full-length Characterization of Proteins in Human Populations Clinical Chemistry. Feb, 2010 | Pubmed ID: 19926773 Glycosylation Status of Vitamin D Binding Protein in Cancer Patients Protein Science : a Publication of the Protein Society. Oct, 2009 | Pubmed ID: 19642159 Population Studies of Intact Vitamin D Binding Protein by Affinity Capture ESI-TOF-MS Journal of Biomolecular Techniques : JBT. Jul, 2008 | Pubmed ID: 19137103 Population Studies of Vitamin D Binding Protein Microheterogeneity by Mass Spectrometry Lead to Characterization of Its Genotype-dependent O-glycosylation Patterns Journal of Proteome Research. Sep, 2008 | Pubmed ID: 18686987 Detection of Bumetanide in an Over-the-counter Dietary Supplement Journal of Analytical Toxicology. Nov-Dec, 2007 | Pubmed ID: 18093421 Analysis of a Challenging Subset of World Anti-Doping Agency-banned Steroids and Antiestrogens by LC-MS-MS Journal of Analytical Toxicology. Apr, 2007 | Pubmed ID: 17579958 Concept for Facilitating Analyst-mediated Interpretation of Qualitative Chromatographic-mass Spectral Data: an Alternative to Manual Examination of Extracted Ion Chromatograms Analytical Chemistry. Jul, 2007 | Pubmed ID: 17542552 Algorithm-assisted Elucidation of Disulfide Structure: Application of the Negative Signature Mass Algorithm to Mass-mapping the Disulfide Structure of the 12-cysteine Transforming Growth Factor Beta Type II Receptor Extracellular Domain Analytical Biochemistry. Jun, 2004 | Pubmed ID: 15136171 The Effect of Hair Color on the Incorporation of Codeine into Human Hair Journal of Analytical Toxicology. Nov-Dec, 2003 | Pubmed ID: 14670132 Automated Data Interpretation Based on the Concept of "negative Signature Mass" for Mass-mapping Disulfide Structures of Cystinyl Proteins Journal of the American Society for Mass Spectrometry. Sep, 2003 | Pubmed ID: 12954171 Recognition of Cysteine-containing Peptides Through Prompt Fragmentation of the 4-dimethylaminophenylazophenyl-4'-maleimide Derivative During Analysis by MALDI-MS Protein Science : a Publication of the Protein Society. Jul, 2003 | Pubmed ID: 12824503 Ofloxacin As a Reference Marker in Hair of Various Colors Journal of Analytical Toxicology. Apr, 2003 | Pubmed ID: 12731656 Cocaine, Benzoylecgonine, Amphetamine, and N-acetylamphetamine Binding to Melanin Subtypes Journal of Analytical Toxicology. Apr, 2003 | Pubmed ID: 12731652 Mass Mapping Sites of Nitration in Tyrosine Hydroxylase: Random Vs Selective Nitration of Three Tyrosine Residues Chemical Research in Toxicology. Apr, 2003 | Pubmed ID: 12703971 Dopamine Biosynthesis is Regulated by S-glutathionylation. Potential Mechanism of Tyrosine Hydroxylast Inhibition During Oxidative Stress The Journal of Biological Chemistry. Dec, 2002 | Pubmed ID: 12376535 Influx and Efflux of Amphetamine and N-acetylamphetamine in Keratinocytes, Pigmented Melanocytes, and Nonpigmented Melanocytes Journal of Pharmaceutical Sciences. Jun, 2002 | Pubmed ID: 12115851 Evaluación del estrés oxidativo en muestras biológicas utilizando el ensayo de sustancias reactivas al ácido tiobarbitúrico Jesús Aguilar Diaz De Leon1, Chad R. Borges 1School of Molecular Sciences, The Biodesign Institute - Center for Personalized Diagnostics, Arizona State University JoVE 61122 Química El análisis de glicanos Nodo: un enfoque de abajo hacia arriba para Glicómica Sahba Zaare1, Jesús S. Aguilar1, Yueming Hu1, Shadi Ferdosi1, Chad R. Borges1 1Department of Chemistry & Biochemistry, The Biodesign Institute – Center for Personalized Diagnostics, Arizona State University Química
Evaluación del estrés oxidativo en muestras biológicas utilizando el ensayo de sustancias reactivas al ácido tiobarbitúrico Jesús Aguilar Diaz De Leon1, Chad R. Borges 1School of Molecular Sciences, The Biodesign Institute - Center for Personalized Diagnostics, Arizona State University JoVE 61122 Química
El análisis de glicanos Nodo: un enfoque de abajo hacia arriba para Glicómica Sahba Zaare1, Jesús S. Aguilar1, Yueming Hu1, Shadi Ferdosi1, Chad R. Borges1 1Department of Chemistry & Biochemistry, The Biodesign Institute – Center for Personalized Diagnostics, Arizona State University Química