Stephen Lye Department of Physiology University of Toronto Biography Publications Institution JoVE Articles Stephen Lye has not added a biography. If you are Stephen Lye and would like to personalize this page please email our Author Liaison for assistance. Publications Glucocorticoids Modulate Multidrug Resistance Transporters in the First Trimester Human Placenta Journal of Cellular and Molecular Medicine. Apr, 2018 | Pubmed ID: 29691980 Maternal Blood Endotoxin Activity in Pregnancies Complicated by Preterm Premature Rupture of Membranes The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians. Apr, 2018 | Pubmed ID: 29656690 Reply American Journal of Obstetrics and Gynecology. Mar, 2018 | Pubmed ID: 29604285 Nurturing Care for Young Children Under Conditions of Fragility and Conflict New Directions for Child and Adolescent Development. Mar, 2018 | Pubmed ID: 29537179 Extracellular Vesicle RNAs Reflect Placenta Dysfunction and Are a Biomarker Source for Preterm Labour Journal of Cellular and Molecular Medicine. May, 2018 | Pubmed ID: 29516617 Chorioamnionitis Induces a Specific Signature of Placental ABC Transporters Associated with an Increase of MiR-331-5p in the Human Preterm Placenta Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology. 2018 | Pubmed ID: 29402780 Comparative Analysis of Gene Expression in Maternal Peripheral Blood and Monocytes During Spontaneous Preterm Labor American Journal of Obstetrics and Gynecology. Mar, 2018 | Pubmed ID: 29305255 Differential Expression of Myometrial AP-1 Proteins During Gestation and Labour Journal of Cellular and Molecular Medicine. Jan, 2018 | Pubmed ID: 28945005 Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-parametric Flow Cytometry Methods in Molecular Biology (Clifton, N.J.). 2018 | Pubmed ID: 28434167 HoxA10 and HoxA11 Regulate the Expression of Contraction-Associated Proteins and Contribute to Regionalized Myometrium Phenotypes in Women Reproductive Sciences (Thousand Oaks, Calif.). Jan, 2018 | Pubmed ID: 28372536 Progesterone Via Its Type-A Receptor Promotes Myometrial Gap Junction Coupling Scientific Reports. Oct, 2017 | Pubmed ID: 29042599 Maternal Vascular Malperfusion and Adverse Perinatal Outcomes in Low-Risk Nulliparous Women Obstetrics and Gynecology. Nov, 2017 | Pubmed ID: 29016509 Activated NK Cells Cause Placental Dysfunction and Miscarriages in Fetal Alloimmune Thrombocytopenia Nature Communications. 08, 2017 | Pubmed ID: 28794456 Prioritizing Research for Integrated Implementation of Early Childhood Development and Maternal, Newborn, Child and Adolescent Health and Nutrition Platforms Journal of Global Health. Jun, 2017 | Pubmed ID: 28685048 Peripheral Maternal Leukocytes Are Activated in Response to Cytokines Secreted by Uterine Tissues of Pregnant Women Cellular & Molecular Immunology. Jul, 2017 | Pubmed ID: 28603281 Immunophenotyping and Activation Status of Maternal Peripheral Blood Leukocytes During Pregnancy and Labour, Both Term and Preterm Journal of Cellular and Molecular Medicine. Oct, 2017 | Pubmed ID: 28429508 Definitive Class I Human Leukocyte Antigen Expression in Gestational Placentation: HLA-F, HLA-E, HLA-C, and HLA-G in Extravillous Trophoblast Invasion on Placentation, Pregnancy, and Parturition American Journal of Reproductive Immunology (New York, N.Y. : 1989). 06, 2017 | Pubmed ID: 28185362 Nurturing Care: Promoting Early Childhood Development Lancet (London, England). 01, 2017 | Pubmed ID: 27717615 Early Childhood Development: the Foundation of Sustainable Development Lancet (London, England). 01, 2017 | Pubmed ID: 27717607 Human DNK Cell Function is Differentially Regulated by Extrinsic Cellular Engagement and Intrinsic Activating Receptors in First and Second Trimester Pregnancy Cellular & Molecular Immunology. Feb, 2017 | Pubmed ID: 26277900 Dual Energy X-Ray Absorptiometry Compared with Anthropometry in Relation to Cardio-Metabolic Risk Factors in a Young Adult Population: Is the 'Gold Standard' Tarnished? PloS One. 2016 | Pubmed ID: 27622523 Preeclampsia is Associated with Low Placental Transthyretin Levels Taiwanese Journal of Obstetrics & Gynecology. Jun, 2016 | Pubmed ID: 27343320 Maternal Whole Blood Gene Expression at 18 and 28 Weeks of Gestation Associated with Spontaneous Preterm Birth in Asymptomatic Women PloS One. 2016 | Pubmed ID: 27333071 Matrix Metalloproteinase Expression in the Rat Myometrium During Pregnancy, Term Labor, and Postpartum Biology of Reproduction. 07, 2016 | Pubmed ID: 27251092 Molecular Evidence of Functional Progesterone Withdrawal in Human Myometrium Nature Communications. May, 2016 | Pubmed ID: 27220952 HoxA13 Stimulates Myometrial Cells to Secrete IL-1β and Enhance the Expression of Contraction-Associated Proteins Endocrinology. 05, 2016 | Pubmed ID: 26982635 Summary and Synthesis of Current Knowledge Early Human Development. Mar, 2016 | Pubmed ID: 26852165 Introductory Editorial: Effects of Maternal Physical Activity on the Fetus Early Human Development. Mar, 2016 | Pubmed ID: 26846466 Static Mechanical Loading Influences the Expression of Extracellular Matrix and Cell Adhesion Proteins in Vaginal Cells Derived From Premenopausal Women With Severe Pelvic Organ Prolapse Reproductive Sciences (Thousand Oaks, Calif.). 08, 2016 | Pubmed ID: 26823071 Impact of Maternal Physical Activity on Fetal Breathing and Body Movement--A Review Early Human Development. Mar, 2016 | Pubmed ID: 26811196 Characterization and Novel Analyses of Acute Stress Response Patterns in a Population-based Cohort of Young Adults: Influence of Gender, Smoking, and BMI Stress (Amsterdam, Netherlands). 2016 | Pubmed ID: 26809721 Hypothalamic-pituitary-adrenal Axis Activity Under Resting Conditions and Cardiovascular Risk Factors in Adolescents Psychoneuroendocrinology. Apr, 2016 | Pubmed ID: 26802599 Comparative Characterization of Vaginal Cells Derived From Premenopausal Women With and Without Severe Pelvic Organ Prolapse Reproductive Sciences (Thousand Oaks, Calif.). 07, 2016 | Pubmed ID: 26763525 Elevated MRNA Expression of PGF2α Receptor Splice Variant 2(FP-V2) in Human Decidua is Associated with Incomplete Mifepristone-misoprostol-induced Early Medical Abortion by Regulation of Interleukin-8 The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians. Nov, 2016 | Pubmed ID: 26744271 Changes in Vascular Extracellular Matrix Composition During Decidual Spiral Arteriole Remodeling in Early Human Pregnancy Histology and Histopathology. May, 2016 | Pubmed ID: 26602431 To Serve and to Protect: the Role of Decidual Innate Immune Cells on Human Pregnancy Cell and Tissue Research. Jan, 2016 | Pubmed ID: 26572540 In Vivo Evidence of the Androgen Receptor in Association With Myometrial Cell Proliferation and Apoptosis Reproductive Sciences (Thousand Oaks, Calif.). Feb, 2016 | Pubmed ID: 26342051 Quantitative Large Scale Gene Expression Profiling from Human Stem Cell Culture Micro Samples Using Multiplex Pre-amplification Systems Biology in Reproductive Medicine. 2016 | Pubmed ID: 26237078 Improving Customized Fetal Biometry by Longitudinal Modelling The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians. 2016 | Pubmed ID: 26169714 HoxA13 Regulates Phenotype Regionalization of Human Pregnant Myometrium The Journal of Clinical Endocrinology and Metabolism. Dec, 2015 | Pubmed ID: 26485220 Impact of Bacterial and Viral Challenge on Multidrug Resistance in First- and Third-trimester Human Placenta The American Journal of Pathology. Jun, 2015 | Pubmed ID: 25963552 Stretch-induced Human Myometrial Cytokines Enhance Immune Cell Recruitment Via Endothelial Activation Cellular & Molecular Immunology. Mar, 2015 | Pubmed ID: 24882387 Identification of a Novel Neutrophil Population: Proangiogenic Granulocytes in Second-trimester Human Decidua Journal of Immunology (Baltimore, Md. : 1950). Sep, 2014 | Pubmed ID: 25135830 Leukocyte Driven-decidual Angiogenesis in Early Pregnancy Cellular & Molecular Immunology. Nov, 2014 | Pubmed ID: 25066422 Genome Wide Association Identifies Common Variants at the SERPINA6/SERPINA1 Locus Influencing Plasma Cortisol and Corticosteroid Binding Globulin PLoS Genetics. Jul, 2014 | Pubmed ID: 25010111 Inhibition of Infection-mediated Preterm Birth by Administration of Broad Spectrum Chemokine Inhibitor in Mice Journal of Cellular and Molecular Medicine. Sep, 2014 | Pubmed ID: 24894878 Connexin31.1 (Gjb5) Deficiency Blocks Trophoblast Stem Cell Differentiation and Delays Placental Development Stem Cells and Development. Nov, 2014 | Pubmed ID: 24866916 A New Role for Monocytes in Modulating Myometrial Inflammation During Human Labor Biology of Reproduction. Jul, 2014 | Pubmed ID: 24829032 Investigation of Genetic Variants, Birthweight and Hypothalamic-pituitary-adrenal Axis Function Suggests a Genetic Variant in the SERPINA6 Gene is Associated with Corticosteroid Binding Globulin in the Western Australia Pregnancy Cohort (Raine) Study PloS One. 2014 | Pubmed ID: 24691024 Expression of Extracellular Matrix-remodeling Proteins is Altered in Vaginal Tissue of Premenopausal Women with Severe Pelvic Organ Prolapse Reproductive Sciences (Thousand Oaks, Calif.). Jun, 2014 | Pubmed ID: 24343133 Association of a Body Mass Index Genetic Risk Score with Growth Throughout Childhood and Adolescence PloS One. 2013 | Pubmed ID: 24244521 Development and Validation of Primary Human Myometrial Cell Culture Models to Study Pregnancy and Labour BMC Pregnancy and Childbirth. 2013 | Pubmed ID: 23445904 The All Our Babies Pregnancy Cohort: Design, Methods, and Participant Characteristics BMC Pregnancy and Childbirth. 2013 | Pubmed ID: 23445747 DREAM Mediated Regulation of GCM1 in the Human Placental Trophoblast PloS One. 2013 | Pubmed ID: 23300953 Expression and Function of Myometrial PSF Suggest a Role in Progesterone Withdrawal and the Initiation of Labor Molecular Endocrinology (Baltimore, Md.). Aug, 2012 | Pubmed ID: 22669741 Common Variants at 12q15 and 12q24 Are Associated with Infant Head Circumference Nature Genetics. Apr, 2012 | Pubmed ID: 22504419 Expression of Genes Encoding Smooth Muscle Contractile Proteins in Vaginal Tissue of Women with and Without Pelvic Organ Prolapse Neurourology and Urodynamics. Jan, 2012 | Pubmed ID: 22038928 An Integrated Systems Biology Approach to the Study of Preterm Birth Using "-omic" Technology--a Guideline for Research BMC Pregnancy and Childbirth. Oct, 2011 | Pubmed ID: 21992798 Consensus PP1 Binding Motifs Regulate Transcriptional Corepression and Alternative RNA Splicing Activities of the Steroid Receptor Coregulators, P54nrb and PSF Molecular Endocrinology (Baltimore, Md.). Jul, 2011 | Pubmed ID: 21566083 Association of Genetic Loci with Glucose Levels in Childhood and Adolescence: a Meta-analysis of over 6,000 Children Diabetes. Jun, 2011 | Pubmed ID: 21515849 Expression of Bone Morphogenetic Protein-1 in Vaginal Tissue of Women with Severe Pelvic Organ Prolapse American Journal of Obstetrics and Gynecology. Jun, 2011 | Pubmed ID: 21397208 Nodal Signals Through Activin Receptor-like Kinase 7 to Inhibit Trophoblast Migration and Invasion: Implication in the Pathogenesis of Preeclampsia The American Journal of Pathology. Mar, 2011 | Pubmed ID: 21356369 All Our Babies Cohort Study: Recruitment of a Cohort to Predict Women at Risk of Preterm Birth Through the Examination of Gene Expression Profiles and the Environment BMC Pregnancy and Childbirth. Dec, 2010 | Pubmed ID: 21192811 Characterization of the Myometrial Transcriptome and Biological Pathways of Spontaneous Human Labor at Term Journal of Perinatal Medicine. Nov, 2010 | Pubmed ID: 20629487 LOX Family Enzymes Expression in Vaginal Tissue of Premenopausal Women with Severe Pelvic Organ Prolapse International Urogynecology Journal. Nov, 2010 | Pubmed ID: 20559617 Vascular-leukocyte Interactions: Mechanisms of Human Decidual Spiral Artery Remodeling in Vitro The American Journal of Pathology. Aug, 2010 | Pubmed ID: 20558572 Expression and Regulation of Prostaglandin Receptors in the Human Placenta and Fetal Membranes at Term and Preterm Reproduction, Fertility, and Development. 2010 | Pubmed ID: 20450832 Developmental Regulation of Cardiovascular Function is Dependent on Both Genotype and Environment American Journal of Physiology. Heart and Circulatory Physiology. Dec, 2009 | Pubmed ID: 19783772 P54nrb is a Transcriptional Corepressor of the Progesterone Receptor That Modulates Transcription of the Labor-associated Gene, Connexin 43 (Gja1) Molecular Endocrinology (Baltimore, Md.). Aug, 2009 | Pubmed ID: 19423654 Transcriptional Activity of Androgen Receptor is Modulated by Two RNA Splicing Factors, PSF and P54nrb Molecular and Cellular Biology. Jul, 2007 | Pubmed ID: 17452459 Fetal Signals and Parturition The Journal of Obstetrics and Gynaecology Research. Dec, 2005 | Pubmed ID: 16343248 Effect of Labor on Glucocorticoid Receptor (GR(Total), GRalpha, and GRbeta) Proteins in Ovine Intrauterine Tissues Journal of the Society for Gynecologic Investigation. Apr, 2003 | Pubmed ID: 12699875 Hypoxia-inducible Factor-1 Mediates the Biological Effects of Oxygen on Human Trophoblast Differentiation Through TGFbeta(3) The Journal of Clinical Investigation. Mar, 2000 | Pubmed ID: 10712429 Inhibition of TGF-beta 3 Restores the Invasive Capability of Extravillous Trophoblasts in Preeclamptic Pregnancies The Journal of Clinical Investigation. Jun, 1999 | Pubmed ID: 10377170 Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae Tali Farine1,2, Michael Parsons2, Stephen Lye1,2,3, Oksana Shynlova1,2,3 1Department of Physiology, University of Toronto, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System, 3Department of Obstetrics and Gynecology, University of Toronto JoVE 57443 Medicina
Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae Tali Farine1,2, Michael Parsons2, Stephen Lye1,2,3, Oksana Shynlova1,2,3 1Department of Physiology, University of Toronto, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System, 3Department of Obstetrics and Gynecology, University of Toronto JoVE 57443 Medicina