Thomas M. Wishart Division of Neurobiology, The Roslin Institute and Royal (Dick) School of Veterinary Studies University of Edinburgh Biography Publications Institution JoVE Articles Thomas M. Wishart has not added a biography. If you are Thomas M. Wishart and would like to personalize this page please email our Author Liaison for assistance. Publications Loss of Glial Neurofascin155 Delays Developmental Synapse Elimination at the Neuromuscular Junction The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Sep, 2014 | Pubmed ID: 25232125 Label-free Quantitative Proteomic Profiling Identifies Disruption of Ubiquitin Homeostasis As a Key Driver of Schwann Cell Defects in Spinal Muscular Atrophy Journal of Proteome Research. Nov, 2014 | Pubmed ID: 25151848 Increased Levels of UCHL1 Are a Compensatory Response to Disrupted Ubiquitin Homeostasis in Spinal Muscular Atrophy and Do Not Represent a Viable Therapeutic Target Neuropathology and Applied Neurobiology. Dec, 2014 | Pubmed ID: 25041530 The Rat Striatum Responds to Nigro-striatal Degeneration Via the Increased Expression of Proteins Associated with Growth and Regeneration of Neuronal Circuitry Proteome Science. 2014 | Pubmed ID: 24834013 A Novel Mouse Model of Warburg Micro Syndrome Reveals Roles for RAB18 in Eye Development and Organisation of the Neuronal Cytoskeleton Disease Models & Mechanisms. Apr, 2014 | Pubmed ID: 24764192 Dysregulation of Ubiquitin Homeostasis and β-catenin Signaling Promote Spinal Muscular Atrophy The Journal of Clinical Investigation. Apr, 2014 | Pubmed ID: 24590288 Label-free Proteomics Identifies Calreticulin and GRP75/Mortalin As Peripherally Accessible Protein Biomarkers for Spinal Muscular Atrophy Genome Medicine. Oct, 2013 | Pubmed ID: 24134804 Total Protein Analysis As a Reliable Loading Control for Quantitative Fluorescent Western Blotting PloS One. 2013 | Pubmed ID: 24023619 Combining Comparative Proteomics and Molecular Genetics Uncovers Regulators of Synaptic and Axonal Stability and Degeneration in Vivo PLoS Genetics. 2012 | Pubmed ID: 22952455 WldS Prevents Axon Degeneration Through Increased Mitochondrial Flux and Enhanced Mitochondrial Ca2+ Buffering Current Biology : CB. Apr, 2012 | Pubmed ID: 22425157 Using Induced Pluripotent Stem Cells (iPSC) to Model Human Neuromuscular Connectivity: Promise or Reality? Journal of Anatomy. Feb, 2012 | Pubmed ID: 22133357 Reversible Molecular Pathology of Skeletal Muscle in Spinal Muscular Atrophy Human Molecular Genetics. Nov, 2011 | Pubmed ID: 21840928 ApoE Isoform-specific Regulation of Regeneration in the Peripheral Nervous System Human Molecular Genetics. Jun, 2011 | Pubmed ID: 21478199 Induction of Cell Stress in Neurons from Transgenic Mice Expressing Yellow Fluorescent Protein: Implications for Neurodegeneration Research PloS One. 2011 | Pubmed ID: 21408118 Synaptic Protection in the Brain of WldS Mice Occurs Independently of Age but is Sensitive to Gene-dose PloS One. 2010 | Pubmed ID: 21124744 SMN Deficiency Disrupts Brain Development in a Mouse Model of Severe Spinal Muscular Atrophy Human Molecular Genetics. Nov, 2010 | Pubmed ID: 20705736 Expression of the Neuroprotective Slow Wallerian Degeneration (WldS) Gene in Non-neuronal Tissues BMC Neuroscience. 2009 | Pubmed ID: 20015399 Molecular Correlates of Axonal and Synaptic Pathology in Mouse Models of Batten Disease Human Molecular Genetics. Nov, 2009 | Pubmed ID: 19640925 Protein Product of CLN6 Gene Responsible for Variant Late-onset Infantile Neuronal Ceroid Lipofuscinosis Interacts with CRMP-2 Journal of Neuroscience Research. Jul, 2009 | Pubmed ID: 19235893 Loss of Translation Elongation Factor (eEF1A2) Expression in Vivo Differentiates Between Wallerian Degeneration and Dying-back Neuronal Pathology Journal of Anatomy. Dec, 2008 | Pubmed ID: 19094180 Modified Cell Cycle Status in a Mouse Model of Altered Neuronal Vulnerability (slow Wallerian Degeneration; Wlds) Genome Biology. 2008 | Pubmed ID: 18570652 VCP Binding Influences Intracellular Distribution of the Slow Wallerian Degeneration Protein, Wld(S) Molecular and Cellular Neurosciences. Jul, 2008 | Pubmed ID: 18468455 Design of a Novel Quantitative PCR (QPCR)-based Protocol for Genotyping Mice Carrying the Neuroprotective Wallerian Degeneration Slow (Wlds) Gene Molecular Neurodegeneration. 2007 | Pubmed ID: 17971231 Differential Proteomics Analysis of Synaptic Proteins Identifies Potential Cellular Targets and Protein Mediators of Synaptic Neuroprotection Conferred by the Slow Wallerian Degeneration (Wlds) Gene Molecular & Cellular Proteomics : MCP. Aug, 2007 | Pubmed ID: 17470424 Synaptic Vulnerability in Neurodegenerative Disease Journal of Neuropathology and Experimental Neurology. Aug, 2006 | Pubmed ID: 16896307 Delayed Synaptic Degeneration in the CNS of Wlds Mice After Cortical Lesion Brain : a Journal of Neurology. Jun, 2006 | Pubmed ID: 16738060 The Neuroprotective WldS Gene Regulates Expression of PTTG1 and Erythroid Differentiation Regulator 1-like Gene in Mice and Human Cells Human Molecular Genetics. Feb, 2006 | Pubmed ID: 16403805 The Slow Wallerian Degeneration Protein, WldS, Binds Directly to VCP/p97 and Partially Redistributes It Within the Nucleus Molecular Biology of the Cell. Mar, 2006 | Pubmed ID: 16371511 Progressive Abnormalities in Skeletal Muscle and Neuromuscular Junctions of Transgenic Mice Expressing the Huntington's Disease Mutation The European Journal of Neuroscience. Dec, 2004 | Pubmed ID: 15579164 A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies Samantha L. Eaton1, Maica Llavero Hurtado1, Karla J. Oldknow2, Laura C. Graham1, Thomas W. Marchant1, Thomas H. Gillingwater3,4, Colin Farquharson2, Thomas M. Wishart1,4 1Division of Neurobiology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, 2Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, 3Centre for Integrative Physiology, University of Edinburgh, 4Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh JoVE 52099 Biologia
A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies Samantha L. Eaton1, Maica Llavero Hurtado1, Karla J. Oldknow2, Laura C. Graham1, Thomas W. Marchant1, Thomas H. Gillingwater3,4, Colin Farquharson2, Thomas M. Wishart1,4 1Division of Neurobiology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, 2Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, 3Centre for Integrative Physiology, University of Edinburgh, 4Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh JoVE 52099 Biologia