从冷冻保存的全血样品制备的辐照血细胞培养物的胞质分裂阻滞微核测定

Published: February 23, 2024

doi:

10.3791/200710

Renu Dayal, Elien Beyls, Anne Vral, Ans Baeyens

¹Radiobiology group, Department of Human Structure and Repair,Ghent University

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Keywords: Cytokinesis-block Micronucleus Assay Irradiated Blood Cell Culture Cryopreserved Whole Blood Samples X-ray Doses RPMI Culture Medium Phytohemagglutinin Cytochalasin B PBS Potassium Chloride Fixative Acridine Orange Stain Phosphate Buffer Fluorescent Microscope Micronuclei

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Cytokinesis-Block Micronucleus Assay on Irradiated Blood Cell Culture Prepared from Cryopreserved Whole Blood Samples. J. Vis. Exp. (Pending Publication), e200710, doi:10.3791/200710 (2024).

从冷冻保存的全血样品制备的辐照血细胞培养物的胞质分裂阻滞微核测定

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