Ensaio de Micronúcleo em Bloco de Citocinese em Cultura de Células Sanguíneas Irradiadas Preparadas a partir de Amostras de Sangue Total Criopreservadas

Published: February 23, 2024

doi:

10.3791/200710

Renu Dayal, Elien Beyls, Anne Vral, Ans Baeyens

¹Radiobiology group, Department of Human Structure and Repair,Ghent University

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Keywords: Cytokinesis-block Micronucleus Assay Irradiated Blood Cell Culture Cryopreserved Whole Blood Samples X-ray Doses RPMI Culture Medium Phytohemagglutinin Cytochalasin B PBS Potassium Chloride Fixative Acridine Orange Stain Phosphate Buffer Fluorescent Microscope Micronuclei

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Cytokinesis-Block Micronucleus Assay on Irradiated Blood Cell Culture Prepared from Cryopreserved Whole Blood Samples. J. Vis. Exp. (Pending Publication), e200710, doi:10.3791/200710 (2024).

Ensaio de Micronúcleo em Bloco de Citocinese em Cultura de Células Sanguíneas Irradiadas Preparadas a partir de Amostras de Sangue Total Criopreservadas

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