*To make HEM, mix 1×10L packet of MEM and160ml of 1M HEPES and bring the volume to 8.75 L using distilled water. Set the final PH to 7.4 and store it at 4°C.
Azari, H., Sharififar, S., Rahman, M., Ansari, S., Reynolds, B. A. Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay. J. Vis. Exp. (47), e2457, doi:10.3791/2457 (2011).