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Analyse av Cell Cycle Posisjon i pattedyrceller
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Biology
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JoVE Journal Biology
Analysis of Cell Cycle Position in Mammalian Cells

Analyse av Cell Cycle Posisjon i pattedyrceller

DOI:
10.3791/3491-v

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12:19 min

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January 21, 2012

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, ,
1Department of Biochemistry, Schulich School of Medicine and Dentistry,University of Western Ontario, 2London Regional Cancer Program, Children’s Health Research Institute, and Department of Biochemistry, Schulich School of Medicine and Dentistry,University of Western Ontario

Chapters

  • 00:05Title
  • 01:19Labeling and Fixing of Cells
  • 02:30Denaturing and Staining of Brdu and DNA
  • 04:11Flow Cytometry and Data Analysis
  • 06:10Alternate Protocol for Cell Cycle Analysis in a Mixed Population of Cells
  • 07:35Cell Cycle Distribution Staining Can Be Used for a Variety of Experimental Purposes
  • 11:47Conclusion

Summary

Automatic Translation

Automatic Translation

Bestemme cellesyklus plasseringen av en populasjon av celler, eller forstå hvordan signaler påvirker spredning, kan lett måles ved flowcytometri hjelp av denne protokollen. Vi rapporterer en enkel eksperimentell tilnærming til flekker celler og kvantifisering av sin posisjon i cellesyklus.

Tags

Cell Cycle PositionMammalian CellsCell ProliferationCell Cycle PhasesDNA SynthesisGrowth RegulationPropidium IodideFlow CytometryRadioactive ThymidineBromodeoxyuridineFluorodeoxyuridineBrdU Staining

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