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Chapters
- 00:05Title
- 02:09Sample Preparation and RNA Extraction
- 03:42Quantitative Reverse Transcription PCR (qRT-PCR)
- 06:48Preparation of Total Histones
- 09:48HPLC Analysis of Linker Histones
- 10:53Representative Results
- 13:26Conclusion
We describe a set of assays to analyze expression levels of H1 linker histones. mRNA of individual H1 genes are quantitatively measured by random primer based reverse transcription followed by real-time PCR, whereas protein quantification of H1 histones is achieved by HPLC analysis.
Tags
Linker-histone SubtypesLinker Histone H1Nucleosome Core ParticleChromatin StructureGene ExpressionMammalian DevelopmentH1 SubtypesSomatic H1sGerm Cell Specific H1 SubtypesH1x5DNA Binding AffinityChromatin Compaction AbilityQuantitative Expression AnalysisMRNA ExpressionReverse Transcription-PCR (qRT-PCR) AssaysNorthern Blot AnalysisHistone Genes
