在大鼠和小鼠的卵巢切除和17β-雌二醇更换：一个可视化示范
Summary
卵巢切除术和硅胶胶囊和口服花生酱，随后更换17β-雌二醇表明，在大鼠和小鼠。
Abstract
雌激素是女性性激素的影响格外广泛的家庭。当大鼠和小鼠使用雌激素的研究，它们通常以消融迅速骑车激素生产，更换外源性17β-雌二醇去势。有，然而，缺乏有关激素应如何管理，以获取生理血清浓度的共识。这是至关重要的，因为17β-雌二醇的水平/管理方法深刻地影响实验结果1-3。我们有一系列特点17β-雌二醇管理的不同模式的研究，发现皮下硅胶胶囊和每口螺母，奶油花生酱均优于市售缓释微丸（创新研究由美国公司生产）在生产生理血清浓度的17β-雌二醇4-6每日注射。当中高级坚果冰淇淋的方法，以前一直使用丁丙诺啡管理7，antages是，当雌激素的管理使用，它类似于口服激素替代疗法和非侵入性。皮下硅胶胶囊是方便和生产最稳定的血药浓度。这部影片的文章包含了卵巢切除术的一步一步的示范和17β-雌二醇激素替代硅橡胶胶囊和口服花生酱在大鼠和小鼠，其次是行政程序的重要方面进行讨论。
Protocol
1。去势通过背路线麻醉动物，例如1.5％异氟醚诱导的（4％），氧/氧化亚氮混合30％/ 70％。将动物俯卧在直肠温度计连接到一个加热板。重要的是有一层薄薄的动物和板块之间的隔离，温度计反馈系统的体温保持在37±0.5°C。申请保护眼睛凝胶和管理5毫克Rimadyl的/公斤体重生理盐水稀释皮下注射动物的脖子上缓解术后疼痛的侧面。 剃一个4 * 4厘米（3×3厘米）对小鼠面积cephally从髂骨?…
Discussion
上面介绍的管理模式，在早先的研究证明优于商业缓释微丸生产生理血清浓度17β-雌二醇注射。而缓释微丸生产非常高，长期的血药浓度峰值，注射导致极端情况下，每天的激素的波动，需要反复的动物4-6压力处理。选择适当的管理模式的重要性，强调了最近的研究表明，虽然低剂量的17β-雌二醇的管理方案（如在目前的文章中提出的技术）防止脑缺血8-10，17β-雌二醇浓度由生产?…
Declarações
The authors have nothing to disclose.
Acknowledgements
资助这项研究是由林雪平大学和东约特兰省县议会。
Materials
| Name of the reagent | Company | Catalogue number | Comments |
| Rimadyl, 50 mg/mL | Pfizer | 462986 | Postoperative analgesia after ovariectomy. |
| Lubrithal eye gel | Leo Laboratories | Eye protection during surgery. | |
| Jodopax vet | Pharmaxim | Surgery disinfection. | |
| Vicryl 4-0 suture | Johnson & Johnson | Wound suture and ovary ligation. | |
| 17β-estradiol, >98% | Sigma | E8875-1G | In sesame oil for silastic capsules and Nutella. |
| Sesame oil | Sigma | S3547-1L | Dissolving the 17β-estradiol. |
| Silastic tubing, ID/OD: 1.575/3.175 mm | Dow Corning | 508-008 | Production of silastic capsules. |
| Wooden applicator stick | Selefatrade | 12-0783 | Capping of silastic capsules. |
| Nutella | Ferrero | Medium for peroral 17β-estradiol. |
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Tags
Ovariectomy17β-estradiol ReplacementRatsMiceEstrogen ResearchHormone ProductionHormone AdministrationPhysiological Serum ConcentrationsSubcutaneous Silastic CapsulesPer-oral Nut-cream NutellaSlow-release PelletsDaily InjectionsNut-cream MethodEstrogen AdministrationPeroral Hormone Replacement TherapyNon-invasiveVideo Article
Citar este artigo
Ström, J. O., Theodorsson, A., Ingberg, E., Isaksson, I., Theodorsson, E. Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration. J. Vis. Exp. (64), e4013, doi:10.3791/4013 (2012).