Materials
Name | Company | Catalog Number | Comments |
NaCl | Fisher Scientific Co. | S271-3 | |
KCl | Fisher Scientific Co. | P217-500 | |
NaH2PO4•H2O | Sigma Chemical Co. | S-9638 | |
MgCl2•6H2O | Fisher Scientific Co. | M33-500 | |
CaCl2•2H2O | Fisher Scientific Co. | C79-500 | |
D-glucose | Fisher Scientific Co. | D16-500 | |
NaHCO3 | Fisher Scientific Co. | S233-500 | |
Sucrose | Fisher Scientific Co. | S5-500 | |
Potassium D-gluconate | Sigma-Aldrich | G4500 | |
HEPES | Sigma-Aldrich | H4034 | |
Adenosine 5′-triphosphate dipotassium salt dihydrate | Sigma-Aldrich | A8937 | |
Guanosine 5′-triphosphate tris salt | Sigma-Aldrich | G9002 | |
Agarose (ultrapure) | Life Technologies | 15510-027 | Gel pored into sterile Petri dish 6 mm thick layer |
KOH | Sigma-Aldrich | P-6310 | |
NaOH | Sigma-Aldrich | S-5881 | |
Bis-fura-2, hexapotassium salt | Invitrogen | B6810 | Cell impermeant |
Name of Equipment | Company | Catalog Number | Comments |
Microtome | Leica | VT1000S | Tissue slicing |
Cyanoacrylic glue (Roti-Coll1) | Carl Roth GmbH+Co | Art-Nr. 0258.1 | |
Microelectrode puller | Narshige International USA | PP-83 | |
Microelectrode Capillary Tubes | World Precision Instruments | 1B150F-4 | |
Microfil 34 g | World Precision Instruments | MF34G-5 | |
Syringe filter | Corning | #431212 | |
Microscope | Leica | DM LFS | With 4X, 40X UV and 63X UV objectives, and epifluorescence |
CCD Camera | Hamamatsu | ORCA ER | 12 bit CCD |
Fura-2 Filter Cube | Chroma | 71500A | Set with UG11 filter |
Polychrome IV | Till Photonics GmBH | Monochronometer | |
Ultraviolet blocking safety glasses | Ultra-Violet Products | ||
EPC-9 amplifier | HEKA Eletronik | ||
Metafluor | Molecular Devices | Imaging Software | |
Patchmaster | HEKA Eletronik | Data Acquisition Software | |
Igor version 6 | Wavemetrics | Electrophysiology & Ca2+ Data Analysis | |
VAPRO 5520 | Westcor | Vapor pressure osmometer |
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