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Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
 
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Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana

Article DOI: 10.3791/1149-v • 11:17 min • August 17th, 2009
August 17th, 2009 •
1, 1, 1
1Crop and Soil Sciences, Cornell University

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This video shows a procedure for isolating intact protoplasts from tissues of 14-day-old seedlings of Arabidopsis. Given that the isolated protoplasts remain intact for at least 96h and are isolated from seedlings instead of one-month-old mature plants, this procedure expedites assays requiring intact protoplasts.

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Isolation Protoplasts Tissues 14-day-old Seedlings Arabidopsis Thaliana Cell Walls Enzymatically Removed Plant Cells Cellular Processes Subcellular Localization Proteins Intact Organelles Targeted Gene-inactivation Double Stranded RNA Interference (RNAi) Protoplast Isolation Protocols Leaf Tissues Mature Arabidopsis Plants Modified Protocols 15 Days Yield Of Protoplasts Time-saving Growth Chamber Space Downstream Studies
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