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Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
 

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence

Article DOI: 10.3791/1694-v • 08:58 min • May 4th, 2011
May 4th, 2011 •
1, 2, 1, 3, 1, 3, 1, 1, 1,4, 3, 2, 1
1Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, 2Department of Biochemistry and Biophysics, University of Pennsylvania, 3Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, 4Departments of Neurosciences and Radiology, University of California

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We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.

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Cerebral Blood Oxygenation Measurement Oxygen-dependent Quenching Of Phosphorescence Spatiotemporal Characteristics Neuro-metabolic-vascular Relationship PO2 Measurement Modalities Larger Fields Of View Higher Spatial Resolution Higher Temporal Resolution Normal Brain Functioning Neurovascular Diseases Stroke Alzheimer's Disease Head Injury Optical Imaging Modalities Hemoglobin Absorption Visible And Near Infrared Range Multispectral Measurement Highly Scattering Brain Tissue Tissue Optical Parameters Blood Oxygenation Estimation Partial Pressure Of Oxygen (pO2) Oxygen-sensitive Dyes
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