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Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
 

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

Article DOI: 10.3791/1726-v • 10:53 min • February 3rd, 2010
February 3rd, 2010 •
1,2,3, 1,2,3, 2,3,4, 1,2,3,4
1Genetics Training Program, University of Wisconsin-Madison, 2Department of Anatomy, University of Wisconsin-Madison, 3Department of Zoology, University of Wisconsin-Madison, 4Cell and Molecular Biology Training Program, University of Wisconsin-Madison

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This protocol describes imaging of individual neurons or neural crest cells in living zebrafish embryos. This method is used to examine cellular behaviors and actin localization using fluorescence confocal time-lapse microscopy.

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Live Imaging Cell Motility Actin Cytoskeleton Individual Neurons Neural Crest Cells Zebrafish Embryos Development In Vivo Optical Clarity High Resolution Imaging Cell Behaviors Migration Axon Outgrowth Guidance Mechanisms Cell Labeling Plasmid DNA Injection Transient Mosaic Expression Pattern Protrusive Activity Molecular Dynamics Transgenic Lines Fine Protrusions Changes In Molecular Distribution
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