Journal
/
Biologia
/
Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus
JoVE Journal
Biologia
Author Produced
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus

Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus

DOI:
10.3791/1944-v

•

09:16 min

•

April 09, 2010

•

, ,
1Department of Chemical and Life Sciences Engineering,Virginia Commonwealth University

Capítulos

  • 00:00Titlle
  • 00:12Introduction
  • 08:26Representative Results
  • 08:42Conclusion
  • 09:02Credits

Summary

Tadução automática

Tadução automática

Suspension immunocytochemical staining of human pluripotent stem cells (hPSCs) for cell-surface markers (SSEA-3/SSEA-4) was achieved based on use of a self-made cytospin apparatus to create a monolayer of cells for observation and quantification.

Tags

Immunocytochemical AnalysisHuman Pluripotent Stem CellsHPSCsHuman Embryonic Stem CellsHESCsHuman Induced Pluripotent Stem CellsHiPSCsSelf-made Cytospin ApparatusCell SourcesSelf-renewal CapabilitiesDifferentiationQPCRIn Vitro DifferentiationIn Vivo DifferentiationIntracellular BiomarkersCell-surface BiomarkersColony GrowthMarker EvaluationMonolayer Cell Samples

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/1944?article_type=v

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
check_url/pt/1944?article_type=v

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
check_url/pt/1944?article_type=v

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
check_url/pt/1944?article_type=v

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP
check_url/pt/1944?article_type=v

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
check_url/pt/1944?article_type=v

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format
check_url/pt/1944?article_type=v

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
check_url/pt/1944?article_type=v

Induction and Analysis of Epithelial to Mesenchymal Transition
check_url/pt/1944?article_type=v

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
check_url/pt/1944?article_type=v

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Read Article