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Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
 
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Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Article DOI: 10.3791/2002-v • 13:10 min • September 8th, 2010
September 8th, 2010 •
1, 1, 1
1Department of Biology, University of Rochester

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This article describes GFP-based fluorescence in vivo assays that separately quantify homologous recombination and nonhomologous end joining in mammalian cells.

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DNA Double-strand Break Repair Mammalian Cells Nonhomologous End Joining NHEJ Homologous Recombination HR Perturbations Premature Aging Tumorigenesis Quantitative Measurement Fluorescent Reporter Constructs GFP Gene I-SceI Endonuclease Induction Of DSBs GFP Positive Cells Flow Cytometry
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