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JoVE Journal Immunology and Infection

Gastric Fluid Sampling, DNA Extraction, and qPCR for H. pylori Detection and its Antibiotic Resistance (Video) | JoVE

Gastric Fluid Sampling, DNA Extraction, and qPCR for H. pylori Detection and its Antibiotic Resistance (Video) | JoVE

qPCR-Based Rapid, Noninvasive Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Article DOI: 10.3791/200247-v • 03:54 min • July 28th, 2023

July 28th, 2023 •

Liang Wang*^1,2,3, Jin-Xin Lai*¹, Yu-Ting Si*^1,4, Xu-Xia Cui^1,4, Zeeshan Umar^1,5, Xiao-Jun Ru¹, Xin-Yu Zhang¹, Zheng-Kang Li¹, Alfred Chin Yen Tay^5,6,7,8, Barry J. Marshall^5,6,7,8, Guang-Hua Li¹, Bing Gu¹

¹Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, ²Division of Microbiology and Immunology, School of Biomedical Sciences, The University of Western Australia, ³Center for Precision Health, School of Medical and Health Sciences, Edith Cowan University, ⁴Medical Technology School of Xuzhou Medical University, ⁵Marshall Laboratory of Biomedical Engineering, School of Medicine, Shenzhen University, ⁶The Marshall Centre for Infectious Diseases Research and Training, The University of Western Australia, ⁷Marshall International Digestive Diseases Hospital, Zhengzhou University, ⁸Marshall Medical Research Center, Fifth Affiliated Hospital of Zhengzhou University

* These authors contributed equally

Tags

Quantitative Polymerase Chain Reaction (qPCR) Rapid Diagnosis Helicobacter Pylori Infection Antibiotic Resistance Gastric Cancer Swallowed String Test Personalized Medicine Precision Medicine Non-invasive Gastric Diseases Diagnostic Time Early Gastric Cancer Screening H.pylori Culturing Technique Sensitive And Specific Test Antibiotic Resistance Profile

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