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Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein (Video) | JoVE
 

Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein (Video) | JoVE

Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles

Article DOI: 10.3791/200736-v • 02:35 min • August 25th, 2023
August 25th, 2023 •
1, 1, 1,2, 1
1Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 2Department of Biochemistry, Faculty of Medicine and Dentistry, University of Alberta

Tags

ABCG5/G8 Lipidic Bicelle X-ray Crystallography Membrane Proteins Lipid Bilayer Detergents Stability Uniformity Structural Characterization Bicelles Phospholipids High-quality Diffraction Crystals High-resolution Structure Native Conformation Functionality Three-dimensional Crystals Detergent-solubilized Heterodimeric ABCG5/G8 DMPC/CHAPSO Bicelles Cholesterol Vapor Diffusion Protein Crystallization
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