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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantitative Measurement of Epithelial Barrier Integrity and Confluency of 2D Bovine Ileal Enteroid-Derived Monolayer (Video) | JoVE
 

Quantitative Measurement of Epithelial Barrier Integrity and Confluency of 2D Bovine Ileal Enteroid-Derived Monolayer (Video) | JoVE

Establishment of a 2D Monolayer from 3D Bovine Ileal Enteroid Culture

Article DOI: 10.3791/200826-v • 01:46 min • April 5th, 2024
April 5th, 2024 •
1, 1, 1
1Department of Farm Animal Medicine, College of Veterinary Medicine, Midwestern University

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Keywords: Bovine Primary Enteroid Two-dimensional Monolayer Culture Translational Biomedical Research Host-pathogen Interactions Immune Cell Dynamics Infection Protocol Transwell Co-culture Tissue Architecture Cryptosporidium Adult Calf Organoid Complexity Tissue Architecture Drug Efficacy Toxicity Tissue Bioengineering Three-dimensional Intestinal Lumen Secreted Substances Luminal Microinjection Mechanical Disruption Apical-out Stromal Cells Pathophysiological Dynamics
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