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Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap
 

Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

Article DOI: 10.3791/2012-v • 05:46 min • April 26th, 2010
April 26th, 2010 •
1, 2, 2, 2
1Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics, Princeton University, 2Department of Physics, Lewis-Sigler Institute for Integrative Genomics, Princeton University

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We present a protocol for bending filamentous bacterial cells attached to a cover-slip surface with an optical trap to measure the cellular bending stiffness.

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Measuring Bending Stiffness Bacterial Cells Optical Trap Protocol Filamentous Rod-shaped Bacteria Forces Microscopic Three-dimensional Spring High-intensity Laser Beam Chemically-treated Coverslip Live Bacteria Growing Ends Filamentous Growth Drug Cephalexin Cell Attachment Bending Forces Polylysine-coated Bead Force Measurement Displacement Measurement
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