Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / In vitro tRNA Methylation Assay with the…
JoVE Journal
Immunology and Infection

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Immunology and Infection
In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
 
Click here for the English version

In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme

Article DOI: 10.3791/2390-v • 12:36 min • October 19th, 2010
October 19th, 2010 •
1, 2, 2, 1
1Faculty of Medicine, Rappaport Institute, Technion - Israel Institute of Technology, 2The Pharmacy and Biochemistry Institute, Johannes Gutenberg University

Capítulos

Resumo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

This protocol describes the preparation of a synthetic tRNA substrate for the Entamoeba histolytica DNA/tRNA methyltransferase 2 (Dnmt2) homolog Ehmeth and the measure of its methyltransferase activity. This experimental approach can be used for investigating the activity of other Dnmt2 proteins.

Tags

In Vitro TRNA Methylation Assay Entamoeba Histolytica DNA Methyltransferase Dnmt2 Ehmeth Enzyme Protozoan Parasites Amebiasis Infectious Agents Clinical Manifestations 5-methylcytosine (5mC) Single DNA Methyltransferase Control Of Repetitive Elements Dual DNA/tRNAAsp Methyltransferase Activity Functional Significance TRNA Methyltransferase Activity Dnmt2 Proteins
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter