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Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture
 

Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture

Article DOI: 10.3791/2471-v • 16:59 min • March 4th, 2011
March 4th, 2011 •
1, 1, 1, 1, 2, 2, 3, 4, 1
1Department of Medicine, University of Chicago, 2Laboratory of Biological Modeling, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 3Department of Surgery, University of Chicago, 4Diabetes Division, University of Massachusetts

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Novel computer-assisted methods of large-scale procurement and analysis of immunohistochemically stained pancreatic specimens are described: (1) Virtual Slice capture of the entire section; (2) Mass analysis of large-scale data; (3) Reconstruction of 2D Virtual Slices; (4) 3D islet mapping; and (5) Mathematical analysis.

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Computer-assisted Large-scale Visualization Quantification Pancreatic Islet Islet Mass Size Distribution Architecture Endocrine Cells Beta-cells Alpha-cells Delta-cells Exocrine Tissues Pancreas Weight Total Beta-cell Mass Islet Size Distribution Functional Limit Large Animal Pancreata Islet Plasticity Cellular Composition Architecture Species Variation
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