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Immunology and Infection

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JoVE Journal Immunology and Infection
A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures
 
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A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures

Article DOI: 10.3791/2616-v • 05:02 min • February 10th, 2011
February 10th, 2011 •
1, 2, 2, 2, 3, 3, 4
1Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Cente, 2Pasadena, CA, Southern California Permanente Medical Group, 3Detroit, Detroit Medical Center, 4Woburn, MA, AdvanDx

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A rapid protocol for the direct identification of Enterococcus faecalis and other Enterococcus species from a positive blood culture using a Peptide Nucleic Acid fluorescent in situ hybridization assay (PNA FISH).

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Enterococcus Species Blood Cultures Identification Procedure Rapid Test E. Faecalis E. Faecium Ampicillin Resistance Vancomycin Resistance Species Differentiation Therapy Resistance Surveillance E. Faecalis OE PNA FISH Assay Peptide Nucleic Acid Probes Fluorescence In Situ Hybridization Time To Results Species-specific Treatment Multicenter Studies 1.5 Hour Procedure 2.5 Hour Assay Procedure Standard Bacteriology Methods
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