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A Gradient-generating Microfluidic Device for Cell Biology
 
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A Gradient-generating Microfluidic Device for Cell Biology

Article DOI: 10.3791/271-v • 11:05 min • August 30th, 2007
August 30th, 2007 •
1, 1, 1, 1, 1, 1
1Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital

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We describe a protocol for the microfabrication of the gradient-generating microfluidic device that can generate spatial and temporal gradients in well-defined microenvironment. In this approach, the gradient-generating microfluidic device can be used to study directed cell migration, embryogenesis, wound healing, and cancer metastasis.

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Gradient-generating Microfluidic Device Cell Biology Cellular Behavior Microfluidic Platform Fluid Flows High-throughput Experiments Soluble Concentration Gradients PDMS-based Microfluidic Devices Growth Factors Fluorescein Isothyiocyanate (FITC)-dextran Epidermal Growth Factor (EGF) Fibroblasts Cell Migration Cell Motility Cell Tracking Analysis
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