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Isolatie & Karakterisering van Hoechst Lage CD45 Negatief Muis Lung Mesenchymale stamcellen
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
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Isolatie & Karakterisering van Hoechst Lage CD45 Negatief Muis Lung Mesenchymale stamcellen

DOI:
10.3791/3159-v

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16:55 min

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October 26, 2011

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, , , ,
1Charles C. Gates Regenerative Medicine and Stem Cell Biology Program,University of Colorado Denver, 2Department of Medicine,University of Colorado Denver, 3Cancer Center,University of Colorado Denver, 4Webb Waring Institute,University of Colorado Denver

Capítulos

  • 00:05Título
  • 01:49Lung Isolation
  • 04:05Preparation of a Single Cell Suspension from Lung Tissue
  • 07:15Flow Cytometry Analysis to Define and Isolate a Lung MSC Population using Hoechst dye Efflux to Detect a Side Population
  • 13:16Enumeration of Lung MSC using a Colony Forming Assay (CFU-F)
  • 14:49Representative Analysis of Isolated MSCs
  • 16:08Conclusion

Summary

Tadução automática

Tadução automática

In dit artikel tonen we de isolatie van murine inwoner long mesenchymale stamcellen (MSC long), hun expansie, karakterisering en analyse van immunomodulerende eigenschappen.

Tags

IsolationCharacterizationHoechstlow CD45negativeMouse LungMesenchymal Stem CellsRegulatorsRegenerationFibrosisInflammationAngiogenesisTumor FormationInjuryDiseasesPulmonary FibrosisArterial HypertensionTechnologyDefine Set Of MarkersRepeated IsolationWell-characterized Stem Cell PopulationFlow CytometrySpecific Cell TypeFunction

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