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ヘキストの分離および特性評価 CD45マウス肺間葉系幹細胞
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
DOI:

16:55 min

October 26, 2011

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Capítulos

  • 00:05Título
  • 01:49Lung Isolation
  • 04:05Preparation of a Single Cell Suspension from Lung Tissue
  • 07:15Flow Cytometry Analysis to Define and Isolate a Lung MSC Population using Hoechst dye Efflux to Detect a Side Population
  • 13:16Enumeration of Lung MSC using a Colony Forming Assay (CFU-F)
  • 14:49Representative Analysis of Isolated MSCs
  • 16:08Conclusion

Summary

Tadução automática

この記事では、マウスの常駐肺間葉系幹細胞(肺MSC)、それらの拡大、特性評価および免疫調節特性の分析の分離を示しています。

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