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Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor
 

Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor

Article DOI: 10.3791/3404-v • 11:27 min • December 6th, 2011
December 6th, 2011 •
1, 1, 1, 1, 1, 1,2
1Stowers Institute for Medical Research, 2Department of Anatomy and Cell Biology, The University of Kansas School of Medicine

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The vomeronasal organ (VNO) detects intraspecies chemical signals that convey social and reproductive information. We have performed Ca2+ imaging experiments using transgenic mice expressing G-CaMP2 in VNO tissue. This approach allows us to analyze the complicated response patterns of the vomeronasal neurons to large numbers of pheromone stimuli.

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Imaging Neuronal Responses Slice Preparations Vomeronasal Organ Genetically Encoded Calcium Sensor Chemosensory Signals Social Sexual Reproductive Status Species Pheromones Predators Vomeronasal Sensory Neurons Specificity Sensitivity G-protein Coupled Receptors V1R V2R FPR Detection Of Chemosensory Cues Pheromone Information Encoding Response Profiles Stimuli Specific Receptors Neuroepithelia Of VNO Vomer Bones Semi-blind Tubular Structure Vomeronasal Duct Nasal Cavity Dendrites Lumen Part Of VNO Pheromone Cues Contact With Receptors Cell Bodies Of VSNs Form Pseudo-stratified Layers
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