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In Ovo Electroporations of HH Stage 10 Chicken Embryos
 
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In Ovo Electroporations of HH Stage 10 Chicken Embryos

Article DOI: 10.3791/408-v • 13:07 min • November 1st, 2007
November 1st, 2007 •
1, 2, 2
1Department of Molecular Genetics and Cell Biology, University of Chicago, 2Department of Human Genetics, University of Chicago

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Chick in ovo electroporation is a technique which allows genetic manipulation of the avian embryo. Common applications of this technique include functional analysis of genes and putative enhancer elements. This video demonstrates neural tube electroporation in HH 10 chick embryos. Injection technique and proper egg handling are discussed.

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Ovo Electroporations Chicken Embryos Developmental Biology Gene Regulation Gene Function DNA Constructs RNA Constructs Gene Expression Gene Knockdown Reporter Constructs Fate Mapping Gene Regulatory Elements Chick Electroporation Quick And Easy Experiments Inexpensive Experiments Window In Eggshell Embryo Manipulation Visualization Of Embryo DNA Injection Fast Green Dye Injection Platinum Electrodes Electrical Pulses Pulse Generator Egg Storage And Handling
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