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JoVE Journal Bioengineering
High Throughput Single-cell and Multiple-cell Micro-encapsulation
 

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Article DOI: 10.3791/4096-v • 16:19 min • June 15th, 2012
June 15th, 2012 •
1, 1
1Department of Mechanical Engineering, Vanderbilt University

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Combining monodisperse drop generation with inertial ordering of cells and particles, we describe a method to encapsulate a desired number of cells or particles in a single drop at kHz rates. We demonstrate efficiencies twice exceeding those of unordered encapsulation for single- and double-particle drops.

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High Throughput Single-cell Multiple-cell Micro-encapsulation Microfluidic Encapsulation Methods Picoliter-scale Drops Confinement Bulk Fluid Environment High Throughput Screening Cytometry Mass Spectrometry Encapsulate Single Cells Capture Set Number Of Cells Isolation Interactions Between Cells Controlled Sizes Drop Generation Techniques Cell And Particle Ordering Aqueous Particle Suspension Immiscible Fluorocarbon Oil Flow Focusing Nozzle Encapsulation Efficiency Polystyrene Particles Poisson Efficiencies
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