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JoVE Journal Neuroscience
Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography
 

Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography

Article DOI: 10.3791/4142-v • 10:11 min • September 5th, 2012
September 5th, 2012 •
1, 1, 1, 1
1College of Pharmacy, Division of Pharmacology and Toxicology, The University of Texas at Austin

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A method to determine the time course of ethanol concentration in the brains of rats during operant ethanol self-administration is described. Gas chromatography with flame ionization detection is used to quantify ethanol in the dialysate samples, because it has the sensitivity required for the small volumes that are generated.

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Microdialysis Ethanol Operant Self-administration Gas Chromatography Pharmacological Effects Drugs Of Abuse Orally Administered Drugs Intravenously Administered Drugs Pharmacokinetics Brain Dialysate Ethanol Concentrations Microdialysis-behavioral Experiments Stereotaxic Surgery Sample Collection Dialysate Ethanol Analysis Gas Chromatography With Flame-ionization Detection
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