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Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas
 

Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas

Article DOI: 10.3791/4347-v • 11:58 min • November 14th, 2012
November 14th, 2012 •
*1, *1, *1, *1, 1, 1
1Department of Biology, New York University
* These authors contributed equally

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The Drosophila retina is a crystal-like lattice composed of a small number of cell types that are generated in a stereotyped manner 1. Its amenability to sophisticated genetic analysis allows the study of complex developmental programs. This protocol describes dissections and immunohistochemistry of retinas at three discrete developmental stages, with a focus on photoreceptor differentiation.

Tags

Dissection Immunohistochemistry Larval Drosophila Retinas Pupal Drosophila Retinas Adult Drosophila Retinas Compound Eye Ommatidia Cells Cone Cells Pigment Cells Bristle Cell Photoreceptors (PRs) Rhabdomeres Rhodopsins (Rhs) Rh1 R1-R6 PRs R7 PRs R8 PRs Subtypes Larval Eye-antennal Imaginal Disc Epithelial Cells Differentiation Wave Ommatidia Assembly Founder Cell R8 Pupal Development Morphological Changes Rhabdomere Formation Synaptogenesis Rh Expression
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