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分离，培养和成年小鼠Cardiomyoctyes的功能分析
 
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分离，培养和成年小鼠Cardiomyoctyes的功能分析

Article DOI: 10.3791/50289-v • 12:49 min • September 24th, 2013
September 24th, 2013 •
1, 1,2, 1, 1, *1, *1
1Cardiovascular Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Division of Cardiology, Sapienza University
* These authors contributed equally

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在这里，我们描述了使用Langendorff灌流系统成年小鼠cardiomyoctyes隔离。所得到的细胞是钙耐受性，电静态和可培养和转染腺病毒或慢病毒操纵基因的表达。它们的功能也可以使用MMSYS系统和膜片钳技术进行分析。

Tags

细胞生物学，79期，医学，心脏病学，细胞生物学，解剖学，生理学，小鼠，离子通道，原代细胞培养，心脏电生理，成年小鼠心肌细胞，细胞分离，IonOptix，细胞培养，转染腺病毒，膜片钳，荧光纳米传感器
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