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Journal / Neuroscience / Chicken Embryo Spinal Cord Slice Culture Protocol
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Neuroscience

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JoVE Journal Neuroscience
Chicken Embryo Spinal Cord Slice Culture Protocol
 

Chicken Embryo Spinal Cord Slice Culture Protocol

Article DOI: 10.3791/50295-v • 10:57 min • March 25th, 2013
March 25th, 2013 •
*1, *1, 1
1Research Department of Cell and Developmental Biology, University College London
* These authors contributed equally

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Resumo
Automatic Translation

Slice cultures facilitate the manipulation of embryo development by gene and pharmacological perturbations. However, culture conditions must ensure that normal development can proceed within the reduced environment of the slice. We illustrate a protocol that facilitates normal spinal cord development to proceed for at least 24 hr.

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Keywords: Spinal Cord Slice Culture Chicken Embryo Motor Neurons Neurodevelopment Neurotrophic Factors In Vitro Culture Vibratome Slicing Agarose Embedding
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