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Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
 

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Article DOI: 10.3791/50354-v • 10:40 min • September 10th, 2013
September 10th, 2013 •
1, 1, 1, 1, 1, 1
1Biochemistry and Molecular Biology, Colorado State University

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A method is presented for the reconstitution of model nucleosomal arrays from recombinant core histones and tandemly repeated nucleosome positioning DNA. We also describe how sedimentation velocity experiments in the analytical ultracentrifuge, and atomic force microscopy (AFM) are used to monitor the extent of nucleosomal array saturation after reconstitution.

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Assembly Nucleosomal Arrays Recombinant Core Histones Nucleosome Positioning DNA In Vivo Sources In Vitro Reconstitution Compositionally Uniform Precisely Positioned Sedimentation Velocity Experiments Analytical Ultracentrifuge Macromolecules Centrifugal Force Atomic Force Microscopy Quality Control DNA Templates Chromatin Structure Function
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